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For each experimental treatment, the mean CaCO3 content per shell was divided by the corresponding mean shell area (µg/µm2).
For each experimental treatment, the mean mass of CaCO3 per unit area (µg/µm2) was determined by dividing the mean CaCO3 content of shells by the mean shell area.
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We estimate the mean H2O content of the MFF to be 5.19 ± 1.83 wt%, attributed most simply to chemically bound H2O in the MFF.
Similarly, the DIC flux was calculated as the product of annual African precipitation with a) continental rainwater DIC at a pH of 7.4 and 10°C as in Willey et al. [71], and b) its product with the mean CO2 content of precipitation reported in Miotke [73].
The mean GC3 content of viral ORFans is 44.7%, also significantly lower than that of viral non-ORFans (45.9%; p-value = 7.0e-13 7.0e-13
However, the mean H2O2 content in CVF samples after four hours of full exposure to air was still only 23 ± 5 μM, one hundred times lower than maximal aerobic in vitro production (~2 mM).
The mean H2O2 content of CVF samples (n = 16) assayed immediately after sample collection and transfer to the hypoxic glove-box (i.e., after approximately one minute of aerobic exposure) was 12 μM ± 5 μM.
Similarly, the DIC flux was calculated as the product of annual African precipitation with a) continental rainwater DIC at a pH of 7.4 and 10°C as in Willey et al. [ 71], and b) its product with the mean CO2 content of precipitation reported in Miotke [ 73].
The absence of the RFLP marker resulted in an increase of about 2.6% above the family mean for IV (level of unsaturation), and a decrease of about 6.5% below the family mean for C16:0 content (saturated fatty acid).
The presence of the SSR alleles resulted in an increase of 6.8% above the family mean for C18 0 content.
This means that GC3 content affects the expression pattern.
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