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Mean Abs control, where Abs absorbance at 490 nm.
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Percentage inhibition is calculated as (2) % Inhibition = [ Abs control − Abs extract Abs control ] × 100.
The percentage of inhibition was calculated according to the following equation: % inhibition = Abs control − Abs extract Abs control × 100 Quercetin was used as a positive control.
The radical scavenging activity was calculated from the following equation: (1) Abs sample Abs control % of radical scavenging activity 000 = Abs control − Abs sample Abs control × 100.
The percentage cell viability, calculated using Microsoft Excel®, is as follows: % {text{ Cell Viability }} = left[ {{{left( {{text{Mean Abs}}_{text{control}} - {text{Mean Abs}}_{text{test metabolite}} } right)} mathord{left/ {vphantom {{left( {{text{Mean Abs}}_{text{control}} - {text{Mean Abs}}_{text{test metabolite}} } right)} {{text{Mean Abs}}_{text{control}} }}} right.
The percentage of enzyme inhibition was calculated as follows: (3) % Inhibition = [ (Abs control − Abs samples ) Abs control ] × 100.
The ability of the extracts to scavenge DPPH radical was calculated by the following equation: (3) Inhibition = [ (Abs control − Abs sample ) (Abs control ) ] × 100, where (4) Abs control = absorbance of DPPH radical + methanol Abs sample = absorbance of DPPH radical + sample.
The experiments were performed in triplicates and scavenging activity was expressed as percentage inhibition, using the following formula: (1) % Inhibition = [ (Abs control − Abs samples ) Abs control ] × 100.
The radical scavenging activity was calculated from the following equation: (3) % Radical scavenging activity = Abs control − Abs sample Abs control × 100.
The assay was performed in triplicate according to standardized procedure of diprotin A. The percentage of DPP-IV inhibition was calculated as follows: (4) % Inhibition = [ (Abs control − Abs samples ) Abs control ] × 100.
The experiments were performed in triplicate and the α-amylase inhibitory activity was calculated as percentage inhibition, using the formula (2) % Inhibition = [ (Abs control − Abs samples ) Abs control ] × 100.
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