Tsetse challenge was obtained as a product of tsetse density, trypanosome prevalence and the proportion of blood meals obtained from cattle.
In a randomized cross-over design, participants consumed a meal that contained meat obtained from pork (PM) or chicken (CM) as the main source of protein, and which was prepared in a sweet and sour sauce and served with rice (50 g, cooked weight).
Peptone, agar, and soybean meal were obtained from commercial sources.
Meal (200 g), obtained from yellow lupin seeds, was extracted with 700 ml of buffer A. The slurry was centrifuged (15 min at 20000 g ) and the transferase activity was precipitated from the resulting supernatant with ammonium sulfate (50%70%% saturation).
The request sought data for contractors, including the companies and agreements they worked under, the camps or bases at which they were located, the services--such as mail, emergency medical care, or meals--they obtained from the military, their specialty areas, whether they carried weapons, and government contracting personnel associated with them.
The baseline human blood index (Qh,0) is defined as the population-wide mean proportion of blood meals that are obtained from humans (h), rather than animals, before the introduction of any intervention (Ω = 0).
Fresh midguts containing blood meals or trypanosomes were obtained from 570 living flies.
Fly stocks were maintained at room temperature on corn meal-yeast-agar medium obtained from the University of Arizona fly food facility.
In addition, a sample of duck liver, not from the batch used to prepare the meals in question, was obtained from the supplier on June 13 and tested for Campylobacter spp. by using 25 g of sample cultured on Campylobacter Blood-Free Selective Agar Base after enrichment in Bolton broth (Oxoid, Basingstoke, UK).
Reference oat meal material, FAPAS T2234, was obtained from FAPAS (CSL, York, UK).
