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Autophagic vacuoles were labeled with MDC by incubating tumor cells grown on coverslips with 0.05 mM MDC in PBS at 37°C for 10 min. After incubation, cells were washed four times with PBS and immediately analyzed by fluorescence microscopy using an inverted microscope (BX51TRF, Olympus, Japan) equipped with a filter system (excitation, 380 to 420 nm; emission, 450 nm).
Taken together, we have demonstrated that human CD56+ DCs were very close to mDC by both transcriptomic analysis and functional characterization.
This paper proposes a non-phosgene route for the synthesis of methylene diphenyl dicarbamate (MDC) by the methoxycarbonylation of methylene dianiline (MDA) with methyl carbamate (MC).
To evaluate the effects of T-helper (Th 1 and Th2 cytokines on the production of thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) by cultured endometrial epithelial cells (EEC) and endometrial stromal cells (ESC).
Data from this experiment showed that the mitochondria were visibly enlarged and co-localized with MDC by 96 h post-transfection with Akt2 siRNA (Fig. 7C, compared panels ii iv with x xii).
Data is recorded in the MDC by either the midwife or medical staff.
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A Chinese study showed reduction of hexavalent chromium by MDC method by 75.1% (An et al. 2014).
However, CD56+ DCs are distinguished from other mDCs by expressing pDC specific molecules.
We demonstrated that the CD56+ DCs clustered with mDCs by transcriptional profiling at whole genome level.
These results argue that CD56+ DCs are unique comparing other mDCs by maintaining certain pDC characteristic features.
Accordingly, CD56+ DCs functionally resemble mDCs by producing IL-12 upon TLR4 stimulation and priming naïve T cells without prior activation.
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