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The size of trophozoites present in ten microphotographs taken from damaged areas of each liver (40x) was measured as major (MD) and minor diameter (md) using the IMAGE software measure tool, previously calibrated.
Genomic DNA (gDNA) was isolated from overnight cultures grown in LB medium [10 g Bacto-tryptone, 5 g Bacto-yeast, 5 g NaCL, H2 O to 1 liter, pH 7.5] (BD, Sparks, MD) using the Qiagen Genomic DNA isolation kit (Qiagen, Germantown, MD) and following the procedure outlined for bacterial gDNA isolation.
Michael Mommert at Northern Arizona University in Flagstaff and his colleagues studied the asteroid known as 2011 MD using the infrared camera on NASA's Spitzer Space Telescope.
To evaluate the response of tinnitus in Ménière's disease patients (MD) using the Tinnitus Retraining Therapy (TRT).
The HTBH and HsDSS1-HTBH DNA fragments were respectively subcloned into pCMV6-entry-mycDDK plasmids (OriGene, Rockville, MD) using the SgfI and EcoRV sites.
The Tm1631 protein was predicted to be analogous to endonuclease IV despite sharing <10%% sequence identity, and the proposed Tm1631 DNA complex was subjected to 90 ns long classical MD, using the CHARMM simulation package [5].
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None of these bears were weighed, so we estimated body masses (M) using the morphometric equation50 M=equation509G1.60.
Analytical agarose gel (1%) with a 1-kb marker (M) using the PCR product (5 μl) after DpnI digestion.
Adiabatic surface temperatures (AST) are computed for pool diameters 1.13 m and 3.4 m using the fire dynamics simulator.
This yields a palaeoelevation of 5888 m for the Namling site using the Darjeeling enthalpy estimate as a sea level datum and 6065 m using the AP assemblage.
With visual colorimetry, porphyrin can be detected at a level of 1.0 × 10−6 M, whereas the detection limit of porphyrin is 3.0 × 10−8 M using the EIS method.
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