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Figure 6 shows the total MD generation in different slips as a function of layer thickness.

Open image in new window Fig. 7 Total MD generation of a edge b screw, and c mixed type in In0.4Ga0.6N step-graded In0.4Ga0.6N step-gradedthout graded)/GaN heteroepitand usIn0.4Ga0.6N withoutrs.

Two separate MC3 runs with randomly generated starting trees were carried out for 6 M generations each with one cold and three heated chains.

Migration occurs every m generations.

MCMC analyses were run in BEAST for 50 to 200 M generations, sampling parameters and trees every 20 K generations.

2 M generations were discarded as "burn-in" (trees sampled before the likelihood plots reached a plateau) and consensus trees constructed from the returning sample.

For migration, every m generations each process copies its best individual to the buffer of a randomly selected population, and adds any individuals in its own buffer to its population.

Notwithstanding its convenience in terms of data availability, yeast is not optimal for studying subfunctionalization due to its large effective population size, M. Following duplication, neutral (possibly subfunctionalized) alleles take in the order of M generations to reach fixation, thus we would expect the incidence of subfunctionalization to be lower when M is large.

From the two different control region fragments, we assembled an overlapping alignment of sequences comprising 541 characters from all 148 individuals included in this study; the best fitting model of evolution was HKY+G (AIC; 10 M generations; burnin = 1500, the same settings were used for the 16S rRNA sequences).

In most cases, reversion appeared to be stable, i.e., once svt2 plants reverted, displaying a Col-like phenotype in the M 2 generation, their M 3 progeny continued to appear as Col-like plants.

Table 5 Genetic algorithm parameters Population size (p) 15* (number of variables) Crossover probability (p c) 1.00 Mutation probability (p m) 0.01 Generation (g) 200* (number of variables).