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Pyrosequencing reactions were performed on Biotage PyroMark MD, and data were analyzed using PyroMD Software.
The study was conceived by MM, DF and MD and data collection was completed by MM, JH and HH.
Sequence data was filtered using LUCY (JCVI, Rockville, MD), and data assembly performed with Phred/Phrap, using default assembly parameters [ 71- 73].
Genotyping was performed according to manufacturer's recommendations at the USDA Soybean Genomics and Improvement Lab, Beltsville, MD, and data generated for each genotype was used for allele calling using GenomeStudio Software v2011.1 (Illumina, San Diego, CA).
Images were analyzed using Image Pro Plus system (Media Cybernetics, Bethesda, MD), and data were expressed as means and SEMs of the number of EGFP+ cells per square millimeter.
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The analysis uses a Moderate Resolution Imaging Spectroradiometer NDVI time series covering a period of 13 years (2001 2013) at a spatial resolution of 250 m and data on projects completed in 2006 and 2007.
The depth of the studied wellbore is about 4200 m and data obtained from 360 m intervals between 3800 m to 4160 m.
However, we focus on a 7.6 h period, where maximum diving depth was 63 m, and data display pressure transducer drifts of approximately 1.75 m.
The averaging epoch was defined from 500 ms before stimulus onset to stimulus offset (−500 to 1000 ms), and data were baseline-corrected based on a 300 ms pre-stimulus interval.
Averaged waveform analysis was processed with baseline correction from –50 to 0 ms, and data were digitally filtered off-line with a 1-30 Hzeroero phase shift band-pass filter (12 dB down).
θ f (equation 2) is the mean squared deviation between model prediction (B M ) and data (B E ) across the m readouts, n time points and sexperimental conditions (weighted by the total number of data points n g ).
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