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In accordance with the generally accepted secondary structure of MCF [ 1, 2, 13, 23, 27, 41], we defined amino-ends, carboxyl-ends and two hydrophilic regions (n, c, loop2, loop4) facing the cytosol space as NCLOOP24; transmembrane regions consisting of six α-helices as TR; and three hydrophilic regions facing matrix space as LOOP135 (loop3, loop5, loop5) (Additional file 1).
Quantitative real time PCR was performed on RNA extracts from MCF 10A and MCF 7 cells.
According to previous reports about the function of the members of MCF [ 1, 2], we could obtain the pattern of substrate transport of MCF.
MCF-7/DOX and MCF-7 xenograft mice models were established.
Closed symbols, MCF-7/BCRP cells.
Open symbols, MCF-7 cells.
Open bars, MCF-7 cells.
MCF-7, MCF-10A, and 293T cells were obtained from Dr. Kunxin Luo (University of California at Berkeley, Berkeley, CA).
b CLSM images of MCF-7 cells.
Experiments were repeated with MCF-7.
(A) BCRP expression in MCF-7/BCRP cells.
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