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MBs design with other purposes in mind (such as PCR experiments) have a design that facilitates association to its target at high temperatures, rendering them unsuitable for live cell imaging.
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MB: Design is also about elimination?
Another factor to consider in MB design is the choice of quencher molecule.
Most quenchers used in MB design are non-fluorescent dark quenchers.
Fluorescent inorganic semiconductor quantum dot (Qdot) nanocrystals are example of fluorophore reporters that have been used in MB design.
Two knees with a MB design required reoperation, one for persistent joint stiffness and another to treat septic loosening.
Flexibility in MB design can also enable the arm of the stem to participate in both target hybridization and stem formation.
Semiconductor fluorescent Qdots have also been utilized as fluorophore reporter in MB design but to a lesser degree than organic fluorophore dyes.
In addition to the aforementioned quencher molecules, gold nanoparticles (AuNP) have also been used as a quencher molecule in MB design [28].
MB: design and results of study.
For example, MB design was reported by Morse for a tobramycin sensor [ 149].
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