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The optimization was carried out to maximize yield, I2 and MB number values.
The conditions optimized were activation temperature, chemical impregnation ratio (IR) and activation time with % age yield, iodine (I2) and methylene blue (MB) number as the targeted responses.
The present analyses were partially supported by the Italian Ministry of Health 2013 (Young investigator grant to MB, number: GR-2013-02356060) and by the Italian Association for Cancer Research (A.I.R.C). with grant AIRC '5x1000' Ref. n. 12237.
Table 2 Description of the two CPUs and GPUs used Machine 1 Machine 2 CPU Processor name Intel Core2 Duo T9400 Intel Xeon E5220 Speed 2.53 GHz 2.27 GHz Memory 3.48 GB 5.9 GB GPU Name Quadro NVS 160M GTX 285 Global memory 256 MB 1024 MB Number of multiprocessors 1 30 Number of cores 8 240.
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where n is the overall MB numbers in a GOP.
The results presented in Figure 2 shows that when the buffer capacity of the nodes were increased from 5 to 100 MB, the number of message delivered increased substantially, thus, justifying the applicability of buffer capacity of node in computing its delivery probability.
Its genome size (0.916 Mb) and number of protein-coding genes (817) are in the range typical for mutualists.
For genomes larger than 1500 Mb, the number of PCRs required to anchor one genetic marker is approximately equivalent.
This included complete genome sequences of L. lactis strain IL1403 (2.35 Mb, accession number AE005176) and fragments of the genome of strain SK11 (2.43 Mb, Genbank record GI 62464763).
Using an estimation of the C. gigas genome size of about 823 Mb, the number of molecules represented in 20 ng of gDNA was then calculated.
The total length of contigs (5.00 Mb) and number of contigs (7589) corresponded to 13.5% of the whole-genome size (37.05 Mb) and 54.0% of the number of predicted ORFs (14 063), respectively (Fig. 1, Table 1).
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