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Each animal was sacrificed 30 min after the last session of maze testing and its hippocampus was immediately dissected and stored at −80 °C.
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Effect of Bifidobacterium breve strain A1 treatment on cognitive function in AD model mice evaluated by Y maze test and passive avoidance test.
Memory performance was assessed using the passive avoidance and the Morris water maze tests and the spontaneous locomotor activity was assessed using the open field test.
The Morris water maze test and the step-down passive avoidance test were conducted to evaluate the cognitive function of the rats.
The learning and memory was evaluated using elevated plus maze tests and biochemically, the corticosterone levels were measured in the blood serum.
The impact of METH on working memory was evaluated using the Y maze test and revealed significant mnemonic deficit in the rats acutely treated with the drug.
Previous studies have documented a decrease in the ability of neonatal hypothyroid animals to learn and habituate to maze tests, and an increase in spontaneous activity.
We found that administration of B. breve A1 to AD mice reversed the impairment of alternation behavior in a Y maze test and the reduced latency time in a passive avoidance test, indicating that it prevented cognitive dysfunction.
Then, we next explored whether treatment with acetate or non-viable B. breve A1 could ameliorate cognitive dysfunction induced by Aβ25 35 using Y maze test and passive avoidance test.
Chronic stress significantly impaired memory performance (delayed latency time to reach platform in Morris water maze as well as to reach closed arm in elevated plus maze test) and decreased locomotor activity along with sucrose consumption.
The chaperones were found to prevent Aβ42-induced learning and memory impairments, as assessed by the Morris Water Maze test, and reduce Aβ42-induced glia inflammation and neuronal degeneration in rat brains, as probed by fluorescent immunohistochemical analyses.
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