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The remaining 35K E. fetida probes may target non-coding regulatory RNA genes including microRNAs, snoRNAs, siRNAs and piRNAs.
Similar(59)
SiRNA against FXYD6 targeting its non-coding region and negative control siRNA (siRNA-nc) were from Invitrogen.
Reports in [ 14, 24] showed that RNAi silencing of a gene can be accomplished by targeting transcribed non-coding sequences.
This finding suggests that these transcripts may represent non-coding RNAs.
Alternatively, these sequences may be non-coding, intergenic sequences.
Thus, the F-ESTs in question may be non-coding, or may encode previously undescribed proteins.
To determine if the unannotated sequences identified here may be non-coding RNAs, these sequences were queried against the Rfam database of non-coding RNA families [ 21].
We suspect that many of these regions may represent non-coding RNAs or other regulatory RNAs.
Furthermore, a significant part of the isolated transcripts may be non-coding RNAs.
Most of these transcripts do not contain any ORFs and may function as non-coding transcripts similar to OsIPS1.
Thus, for all practical purposes the pure GA-sequences may be considered non-coding for proteins.
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