It is possible that there may be "threshold" of Bmi-1 protein expression, and when this "threshold" is overridden, this protein may start to function as an oncogene.
When the recombinant protein fails to fold into its native state or protein expression exceeds the folding capacity of the ER (Sha et al. 2013), unfolded proteins may start to aggregate, triggering the UPR pathway.
It should be noted that as the optical resolution continues to improve toward around 10 nm and beyond, the physical size of the antibodies or fluorescent proteins may start to become the limiting factor for determining resolution.
Alternatively, protein aggregation may start and proceed, in a relatively uniform temporal spatial pattern, independently at different sites in the brain of AD and PD patients, and thereby produce the observed stereotypic courses [ 52].
But the second stage may start too.
"I may start work on the sequel.
GREAT restaurants may start out that way.
Otherwise, they may start losing faith.
Decay may start even earlier.
That may start to change.
