However, appreciating the magnitude of diversity of HIV-1 strains in Nigeria may require sequencing and analysis of longer gene regions for the identification of prevalent or emerging CRFs.
However, although exome capture appears an attractive option, the large number of variants identified (in excess of 20 000/subject) may require sequencing of additional family members for filtering which will increase the cost and expand the amount of experimental analysis necessary to confirm pathogenicity (Qi et al., 2012; Wooderchak-Donahue et al., 2012).
Accurately determining relationships between strain clusters may also require sequencing of larger portions of the genome.
The construction of informative trees, however, may be computationally intensive and require sequence information from related species that is often not available.
This may require the sequencing of hundreds of cancers with shared biological features, along with early integration of next-generation sequencing platforms into innovative clinical trials to clearly define the added value of personalized genomics to standard clinical cancer care.
Although specific trigger sequences have not been clearly identified in dimeric coiled coils, higher-order coiled coils, as well as other oligomeric multi-protein complexes, may require such sequences to nucleate and direct their assembly.
There are polymorphic positions in other exons, which may require additional sequencing to exclude certain alleles with differences outside exon 2 and 3, depending on the clinical requirement and relevant accreditation guidelines.
For example, the isolated NES of Rev binds to CRM1 much more weakly than does the full-length Rev protein [61], implying that an NES may require flanking sequences to adopt the conformation needed for CRM1 binding.
Thus, detection of heteroplasmic mtDNA variants at frequencies above 10% requires greater 454 coverage than the >30× coverage estimated for the detection of heterozygous nDNA variants [21], [30], and detection of heteroplasmic variants at lower frequencies may require deeper sequencing, coverages estimated to be at least 1,500× for ≥5% [15] and 15,000× to 100,000× for as low as 2% [14], [31].
Consequently, further refinement of the Anopheles gambiae genome annotation may require genome sequences of additional Anopheles species and, still, supporting experimental evidence seems unavoidable.
