Thus, achieving the level of functional and phenotypic DC diversity in distinct compartments of the respiratory tract under normal versus inflammatory conditions may require both differentiation of local monocytes and recruitment from the blood of immature or precursor mDCs and pDCs.
However, there is evidence to suggest that MSCs may not be well suited to the hostile anaerobic environment of the diseased IVD [ 7, 8] so that long term survival and integration within the disc may require pre-differentiation of the MSCs in culture towards a phenotype more representative of native IVD cells.
These are wedding words that require differentiation.
This suggests that autonomous Notch-induced terminal differentiation may require a stronger signal than non-cell autonomous induction of differentiation.
Various infectious/inflammatory disorders such as tuberculosis and fungal infections and granulomatous disorders such as sarcoidosis could be difficult to differentiate radiologically from neoplastic meningitis and may require meningeal biopsy for differentiation.
Moreover, it is possible that our culture conditions, which utilize high levels of FGF10, may promote this less differentiated cellular state, and our data suggests that distal differentiation may require prolonged time in culture, since SFTPC begins to increase after ∼65 days in culture, and continues to increase at 110 days in culture, the latest time we have examined.
Second, Oct4 downregulation during normal trophectoderm differentiation may require epigenetic regulations, which may be positively influenced by the NuRD complex.
The initiation of C2C12 differentiation may require specific cell signaling mediated by glycans such as PPAR, and Notch signaling.
The transition between alternative modes of PcG regulation may require additional signals upon differentiation (and likewise during tumorigenesis), which may include recruitment of additional transcriptional activators and/or competition with PcG antagonists, the tritorax group (trxG) proteins.
Along with the other risk factors (such as depth of invasion or degree of differentiation), PNS may require adjuvant RT along with surgical excision [33, 34].
