Among the "conserved" transcription factors, some seemed to be pathway specific as they were present only in one or two pathways (e.g. CART1, ETF, RUSH-1α, SF1 and SOX9), while others were present in all or most of the pathways and may represent common regulators of conserved transcriptional co-expression among different pathways.
Factorial and correlation network analyses allowed the identification, at 96 h, of transcriptional and metabolite "hubs" which may represent central regulators; these comprised ABA, its carotenoid precursors (violaxanthin and neoxanthin) and the Nxd gene, leading to neoxanthin biosynthesis.
Collectively, our results provide novel evidence that signaling pathways engaged by deregulated RTKs in CRC, including those reliant on Grb2 or Shc, may represent important regulators of anoikis resistance in IECs, a process of outmost relevance in cancer metastasis.
Given that miR-122 already has a history as a circadian output modulator (Gatfield et al., 2009), it is tempting to speculate that the other miRNAs may represent specialised regulators of the rhythmic transcriptome as well.
Also, utilizing heuristically defined thresholds (>2-fold changes in gene expression) to define significance may have limited our ability to recover genes that were more subtly expressed individually, but in combination with other genes, may represent important regulators of NK cell activation.
We previously identified a series of genes encoding platelet transmembrane proteins that may represent novel regulators of human platelet function and have now used the zebrafish model to conduct the first reverse genetics screen in zebrafish of candidate genes identified in systems biology studies of human blood cell elements.
Recently, it was demonstrated that the tissue kallikrein family of proteinases are able to regulate PAR signaling and may represent important endogenous regulators PAR1, PAR2, and PAR4 [ 53].
Recently, it was demonstrated that tissue kallikrein family of proteinases are able to regulate PAR signaling and may represent important endogenous regulators PAR1, PAR2, and PAR4 [ 35].
In addition, because IGF1R is not only an upstream regulator of metabolism (e.g., glycolysis) but also regulates STAT1, miR-145 may represent a key regulator of both metabolism and a signaling pathway (JAK/STAT pathway).
Two of them, PtRR27 and PtRR29, have an atypical conserved motif (HD-D-K and DD-E-K, respectively), and may represent pseudo response regulators.
