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The dominant diploid peaks in FC of BAC may represent contaminated non-cancerous cells in the sample.
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Although the remaining <2% might represent "contaminating non-CM", some of these may have been due to incomplete cell permeabilization and an inability of the anti-cTnT antibody to bind its antigen.
This may occur for the repeat-rich human genome and equally likely alignments can still represent contaminating sequences.
Thus the 2N subpopulations most likely represent contaminating stroma.
Three sequences showed similarity to non-plant proteins and probably represent contaminating sequences (Table 1).
These could represent contaminating sequences that were not filtered prior to transcriptome assembly.
To further refine and validate our data we excluded ORFs that could potentially represent contaminating DNA.
The few Thy1high cells expressing Krt18 may represent either contaminating cells, or cells which are in process of transition towards the Thy1low-state (see below).
However, these candidate microbe genomes were not detected in the samples tested, suggesting that infrequent DK tags matching exogenous genomic sequences may represent rare contaminating DNA sequences which are difficult to amplify, or may result from sequencing errors or variation in human genomic sequences that are identical to microbial sequences.
Clearly, our selection of the first spikes is only a very rough estimate of the spikes potentially representing the onset of the responses and may be "contaminated" by spontaneous spikes that are not elicited by the stimulus.
Because rice is a staple food in Bangladesh, providing > 70% of the daily calorific intake (Ninno and Dorosh 2001), consumption of contaminated rice may represent a significant As exposure pathway.
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