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Previous studies suggested that high-identity duplications (identity > 94%) frequently collapsed within working draft sequence assemblies [ 23] and may represent artificial duplications within an assembly [ 18].
As larger, high-identity duplications (≥ 94%) are frequently collapsed within working draft sequence assemblies [ 28] or may represent artificial duplications within an assembly [ 34], we compared these WGAC results to whole genome shotgun sequence detection (WSSD) results.
As larger, high-identity duplications (the 267.0 Mb unshaded region in Fig. 1) are frequently collapsed within working draft sequence assemblies [ 28] or may represent artificial duplications within an assembly [ 34], we compared these WGAC results to those detected by the assembly-independent WSSD approach.
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Therefore, we asked whether Ser116 may represent an artificial in vitro CTKD target that in vivo is not involved in substrate phosphorylation.
As large, high identity alleles (≥ 99.5%) may not be merged and represent artificial duplications due to local assembly errors [ 34], we excluded all these alignments to eliminate artificial duplications in Batu_4.0.
Not all reads that share the same first 50 bases represent artificial duplication.
The dot lines represent artificial homologous mappings detected by unbalanced Bi-random walk algorithm.
Nevertheless, we acknowledge that this value may represent a rather artificial boundary with viruses.
Moreover, we have previously determined that focal adhesion molecules may represent targets for recent artificial selection in the Thoroughbred and therefore may be critical to the development of the muscle strength phenotype for which Thoroughbreds are renowned [ 55].
Of course, different fusion procedures and different types of artificial discs may represent biases in comparing outcomes.
The finding that exosomes conveying miRNAs are able to cross the blood-brain barrier [ 69] suggests that simple intravenous injection of artificial exosomes may represent an effective way of delivering miRNAs to the ischemic brain.
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