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Several studies suggested that various levels of DNA methylation may regulate tissue-specific transcription [17] and be important for normal development or differentiation [18].
Hormones or abiotic stresses may regulate tissue-specific promoters.
Recent studies have also revealed that hypomethylation occurs at more localized regions, termed DNA methylation valleys (DMVs), which are enriched for developmental genes and may regulate tissue-specific expression [ 20, 25].
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Based on the results from this study and the functions during the gene expression, we think that differences in methylation levels and patterns may be important to regulate tissue-specific gene expression and cell differentiation, however we need to perform further studies to confirm this.
These data identify Sox9 as the first transcription factor to interact with HDACs, and suggest that HDAC may bind to Sox9 to regulate tissue-specific gene expression.
In several studies, various levels of DNA methylation could regulate tissue-specific transcription and may be important during development and differentiation [ 58].
Thus, it is amazing that regulatory mechanism of tissue-specific alternative splicing is evolutionally conserved from nematode to mammal, and regulation though Fox and another tissue-specific RBMs may turn out to be a widespread essential phenomenon to regulate tissue-specific alternative splicing of multiple genes.
Homodimeric interactions through the ETS domain may play a role in cooperative binding to repeated DNA elements [86], whereas heterodimeric interactions might regulate tissue-specific gene expression [87, 88].
BAF can regulate tissue-specific gene expression in C. elegans by binding and occupying specific promoters [26].
Combinations of specific TFs might regulate tissue-specific genes.
The bHLH transcription factors regulate tissue-specific transcription and regulate many developmental pathways [ 1].
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