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While the failure to have detected DAGLα in pyramidal cell somata may reflect technical limitations in available morphological tools, it does highlight the possibility that different parts of a cell employ different pools of DAGLα for generating eCBs.
However, it may reflect technical difficulties with DNA amplification after paraffin embedding of the specimen or a possible later clearance of the virus from infected tissues.
This discrepancy might be due to true biological differences between samples analyzed by the two studies or may reflect technical biases related to the method of analysis.
This may reflect technical difficulties with the TOF-Watch as calibration data were not adequate prior to start of monitoring, with unstable individual twitch responses together with unstable and fluctuating TOF ratio values.
As the method is semiquantitative, the different intensities between the amounts of P1 and P2 precipitated by ZF + KTS may reflect differences in binding affinity or may reflect technical variation.
It is possible that such discrepancies may reflect technical differences in microvesicle isolation but given that α-synuclein is abundant in the circulation and known to easily associate with membranes, caution is required when interpreting candidate-based measurements.
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Although the different number of Atrx binding peaks identified in our mNPC profiles may partially reflect technical variability between the two studies, the notable correlation of Atrx distribution with gene bodies and promoter regions, not seen in mESCs, speaks to a hitherto unappreciated, cell line-specific functionality for Atrx.
The failure to validate the expression microarray results by qRT-PCR results may partially reflect technical differences between the platforms.
The ~15-fold increase in PADI2 expression found in our study, compared to the ~4-fold increase found in the previous study [ 12], may simply reflect technical differences between the studies as we utilized TaqMan qRT-PCR compared to microarray analysis.
First, taking out more lymph nodes may reflect superior technical ability on behalf of the surgeon.
This may reflect the technical difficulties in detecting anti-E1 responses, as the protein misfolds unless co-expressed with E2 (Dubuisson et al., 1994).
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