Several Abi-1 or Abi-2 bands observed for the mouse brain lysates may reflect splicing variants, or the phosphorylation or partial degradation of these proteins.
The position-specific conservation scores of exons and their flanking intronic regions may reflect functional splicing cis elements.
The fusion products are present at very low levels, and thus may reflect promiscuous splicing involving ZC3HAV1L and CHMP1A.
A similar band pattern has recently been observed on Western blotting for KCa3.1 in human fibrocytes, lung fibroblasts and airway smooth muscle cells, and may reflect differential splicing modification [ 22– 22].
As noted earlier, each Locus may include several highly similar transcript isoforms, whose sequence differences could reflect splice variants, homeologs and paralogs, and sequencing errors.
Low validation of specific genes could reflect splice variants.
Data concerning FGF8 correlation with clinical parameters, however, have been conflicting and may reflect differences in splice form utilisation (Leung et al, 1996; Tanaka et al, 1998; Dorkin et al, 1999; Valve et al, 2001).
The high level of alternative splicing may reflect variations in binding affinities of regulatory factors to 3' UTRs influenced by variations in 3' UTR structure or in polyadenylation complex proteins.
Synergy between proteins in splicing may reflect cooperative binding to a functional element(s), or multiple, simultaneous interactions between the activators and other components of the splicing machinery (reviewed in [43]).
Why SNCA levels should be lower in Parkinson disease CSF is not clear, but may reflect alterations in transcription, splicing or processing, or increased clearance.
