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This observation could be interpreted taking into account protein overcrowding on the carrier surface that may reduce substrate accessibility to the active site.
For example, impairment of RDR2 may reduce substrate competition between RDR2 and RDR6 and increase methylation in rdr2 mutants (Jauvion et al. 2012).
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As M. isabellina cannot utilize cellulose and xylan, it is reasonable to speculate that a co-culture with lignocellulolytic fungi such as T. reesei and P. chrysosporium may reduce the substrate cost by increasing the portion of useable materials in the substrate mixture.
This forms a hydrophobic tunnel in combination with two tyrosines, which may reduce the substrate lysine p Ka, such that the neutral amine required for attack is more favored.
In addition, too small particle thickness may result in the adhesion and agglomeration of the solid substrate during SSF, which may reduce the solid substrate porosity and the gas diffusion rate and increase the accumulation of heat.
By analogy to NifEN, these enzymes may be able to reduce substrates with a limited number of electrons such as acetylene and azide [ 28].
We also predict that conditions that mimic natural habitat influence mobility positively, whereas unnatural (no substrate) or adverse conditions (dry substrate) may reduce speed of movement.
Both increased the mRNA expression of lactate dehydrogenase A (LDHA) which metabolites pyruvate to lactate and alanine aminotransferase (ALT) which converts pyruvate to alanine, suggesting that these two pathways may contribute to reducing substrate flux into the TCA cycle and limiting oxidative phosphorylation [ 64].
Others have suggested that nitric oxide substrates may reduce bacterial translocation by preserving microvascular blood flow [ 10].
Furthermore, given that the detected similarities focus on common cofactors and ubiquitous molecules such as ATP, the results also suggest that targeting the sub-pockets of less ubiquitously used substrates may reduce the chance of cross-reactivity.
Fundamentally, earthquakes affect the stability of slopes in two ways: (1) the ground shaking may reduce the frictional strength of the substrate by shattering of rock mass or liquefaction; and (2) the seismic acceleration may result in short-lived and episodic changes of the tensile and shear stresses in the hillslopes during earthquakes.
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