Exact(1)
Furthermore, additional polyadenylation signals may produce transcript isoforms with several combinations of miRNA binding sites, which adds even more complexity [ 39].
Similar(59)
AS producing premature stop codons, such as SVs 2,4,5,6 and 7 of MtSERK3, may produce transcripts that are targets for non-sense mediated RNA decay [ 49].
Finally, we measured the renilla/firefly luciferase ORF ratio by qPCR as in some cases cloning of a 5′UTR between open reading frames may produce alternative transcripts due to differential splicing.
It may produce important transcripts.
Additionally, alternative splicing may produce multiple transcripts from the same locus.
Whether the MsSCP-x/SCP-2 gene may produce two transcripts via alternative splicing needs further investigation.
A single gene may produce several different transcripts, each with its own TSS and corresponding promoter.
There are also limitations to our understanding of the transcriptome and therefore two different probes that, theoretically, detect the same transcript may produce different measurements in microarray experiments because of cross-hybridization from unknown splice variants, gene families, and transcribed pseudogenes.
There are, however, some observations that suggest these alterative transcripts may produce soluble protein.
Therefore, we propose that production of chimeric transcripts may produce active competition with original proteins and produce dominant negative phenotypes in cancer.
As in all RNA-Seq experiments, the variable length of transcripts may produce biases in the data [ 23]; therefore, we analyzed GO enrichment via the Goseq R package, which was specifically designed to limit bias in the data [ 24].
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