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However, during the cell cycle arrest some miRNAs may mediate translational activation of specific transcripts [2].
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These findings further indicate that other factors may contribute to or modulate the functionality of PUM binding sites, for example recognition elements for cofactors like Nanos, which is known to interact with Pumilio to mediate translational repression [55].
In each of these systems, relatively short nascent peptide elements interact with the ribosome exit tunnel to mediate translational arrest.
Good candidates contained long tracts in the appropriate position to mediate translational switching.
Rap55 and Xp54 can mediate translational repression in Xenopus oocytes when tethered to an mRNA [146 148].
However, monitoring of only mRNA changes leaves open the possibility that these sites might still mediate translational repression.
Members of this family can mediate translational repression and mRNA destabilisation in organisms from yeast to vertebrates [110,111].
Argonautes then recruit additional silencing factors that mediate translational repression and degradation of the targeted mRNAs (Huntzinger and Izaurralde, 2011).
miRNAs are short endogenous RNA sequences (∼22 nt) that mediate translational repression of target messenger RNAs (mRNAs) (Bartel 2004).
Interestingly, the RNAi machinery has also been shown to mediate translational repression or induce chromatin modification [ 16].
Yamasaki et al. suggest that these fragments may mediate stress-related translational repression [ 84].
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