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The weaker actin affinity of Cdc8pR121A may make actin filaments more susceptible to severing by cofilin, impair formin (Cdc12p) nucleation of actin filaments for formation of the ring and filaments that are crosslinked by α-actinin or fimbrin, or impair Myo2p function.
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In this view, the functions of Cdc8pD16A.K30A and Fim1p-GFP are synergistic and may make the actin filaments more susceptible to depolymerization or severing by one or more actin binding proteins, such as cofilin-Adf1p.
These proteins are also required to make actin filaments in other parts of the cell.
"We may make progress.
He may make suggestions.
That may make a difference.
That may make political sense.
You may make yourself nervous.
We may make changes".
May make some sense.
"Technology may make stealing easy.
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