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Most azole antifungals inhibit the downstream enzyme lanosterol 14 α-demethylase [7], with the possible exception of abafungin, which may instead affect sterol-C-24-methyltransferase and the fungal cell membrane directly [8].
Thus, this SNP may instead affect TIBC independently of iron status.
Alcohol may instead affect the glyoxylate cycle that bypasses part of the citric acid cycle, including succinate dehydrogenase [ 13, 14].
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This suggests that functional divergence of newly created homedomain proteins does not involve changes to DNA binding specificity, but may instead be affected via changes in other, interaction/activation domains.
Long-tongued bumble bee species, specialized to other flower types, may instead be negatively affected by increased competition from the generalists (e.g. due to nectar-robbing of long-tubed flowers) after oilseed rape flowering has ceased.
If not, what locales are instead affected?
These results suggest that the substitution at position 195 does not alter the structure of the active center, but instead may affect a substrate enzyme interaction.
By constructing strains that express phospho-mutant versions of Chs2, we determined that phosphorylation of Chs2 on S222 does not influence the measured in vitro activity of the enzyme, but instead, may affect amount of protein that is localized to specific sites of cells.
However, the inhibition of Wnt5a did not affect the β-catenin translocation, which may instead be regulated by cadherin-catenin signaling.
Instead, mutations may affect one underlying trait (no pleiotropy), but because of which traits are measured, two may show a change.
Indeed the ALS-FUS mutations may instead result in the production of a more rigid protein which in turn appears to affect its RNA binding properties [ 19, 24].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com