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The data support structural changes that may initiate aggregation or polymerization by the mutant protein.
These underlying weak chemical interactions may not cause a structural change in the polymers but may initiate aggregation of the aliphatic chains, as both the polymers here contain aliphatic side chains, hence creating relocalized areas with density and refractive index different from the initial values.
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This conformational change exposes the NAC domain that can participate in hydrophobic interactions that may initiate the aggregation process.
To follow amyloid formation, a pH jump (at t = 0) was used to initiate aggregation.
Recombinant httex1-51Q protein fused to glutathione S-transferase (GST-Httex1-51Q) was purified from E. coli and trypsinized to remove GST and initiate aggregation as described [79].
250 nM of PreScission protease was added to initiate aggregation.
First, DBeQ binding to VCP might initiate aggregation directly by altering the solubility of VCP.
Fragmented fibril seeds (10% monomer equivalents) were added to 10 μM K18 or K19 monomer to initiate aggregation.
Since oxi-γ-syn may initiate α-syn aggregation [ 15] we examined whether these synuclein isoforms have overlapping intracellular localization.
A recent study suggested that abnormal cofilin aggregation may initiate tau neuropil threads [9].
The trauma-induced increase in structural protein content and aggregation may initiate a cascade of events leading to ALS.
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