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Cosampled compounds may inhibit amplification assays of environmental samples (Alvarez et al. 1995).
The presence of PCR inhibitors in stool samples (e.g., bilirubin, bile salts, and heme in the faeces) may inhibit amplification and limit the usefulness of PCR technique [ 36, 37].
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However, the test often gave a false-negative result because hemoglobin and other components in blood may inhibit PCR amplification (3, 4, 9 ).
Such extraction failure could result from prolonged formalin fixation and paraffin embedding because these techniques can have a profound effect on the molecular arrangement of DNA and may inhibit its amplification and extraction (14 ).
Radiation may reduce the activity of enzymes, DNase inactivation can also damage the polymerase, restriction enzymes may introduce more contaminating DNA, and unbound DNA intercalators inhibit amplification of the intended template [ 56, 58].
These blockers are added to inhibit amplification of the non-mutant allele, permitting specific amplification of the mutant allele.
As such, serotonergic projections may inhibit engagement of the 'aversive amplification' circuit and dysfunction in this projection may contribute to the negative affective bias in mood and anxiety disorders.
An excess of human DNA may hamper the detection of pathogen genomic material or may inhibit the PCR reaction [ 18, 19]; hemoglobin traces may also inhibit PCR-based amplification.
However, PCR amplification of O. tsutsugamushi DNA from blood often lacks sensitivity because some hemoglobin, iron porphyrin, and other factors may inhibit the PCR, although obtaining and processing the blood that avoids the inhibitors is possible (3, 4, 9 ).
Lack of trust may inhibit innovation, too.
I think this business may inhibit you from saying that.
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