Predictions of the mutants' three-dimensional structure suggested that the hydrogen-bond distance between threonine 83 and aspartic acid 92 may influence reaction specificity, which shifted toward hydrolysis in mutants where this distance was shorter than in the wild type, but toward methanolysis where it was longer.
If we consider that these variable genes may influence host specificity, such identified DNA rearrangements in some T3E genes might have a significant role in pathological adaptation of these plant pathogenic bacteria to their hosts.
Alternatively or additionally, constraints with respect to location within the cytoplasm may influence signalling specificity.
The enzymes also require different buffers, which may influence primer specificity.
Furthermore, our EMSA results suggest that these flanking bases may influence the specificity of the binding site for HIF-1.
Using this approach, we examined how the stimulus-response properties of signaling pathways may influence their specificity.
Here we analyze the sequences, structures, and evolution of LPMOs to understand the factors that may influence substrate specificity both within and between these enzyme families.
Interestingly, the ECE-like group of enzymes (ECEL-1, DINE, etc), for which there are no known physiological substrates, are unique in that all have a LNAYY motif, which may influence substrate specificity.
Molecular analysis of leptospiral OMP antigens is important in understanding the antibody response of hosts, because the antigenic diversity may influence the specificity and sensitivity of the serological assay.
Furthermore, inhibitory effects of cluster 5 substitutions on JM antibody binding were associated with HLA-DQB1*0302 expression within HLA-DRB1*04 patients, suggesting that this major type 1 diabetes susceptibility allele may influence the specificity of the IA-2 autoimmune response, in particular to the JM region that may dominate around the time of first appearance of IA-2 autoimmunity.
