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It is found that the environmental differences between the cage and the animal room must be recognized as these differences may influence experimental result.
The mouse itself must be considered, as issues such as the specificity of genetic manipulations, the supplier of the mice, their health, sex, and sensory or motor impairments may influence experimental findings.
Since a given anaesthesia regime may influence experimental outcomes, the choice of a suitable anaesthesia has to be considered carefully in regard to the experimental requirements [ 1],[ 3].
These data underscore the importance of considering the unique behavioral characteristics of different inbred mouse strains that may influence experimental outcomes.
In addition, differences in the histological distribution of vector-specific cellular receptors, such as the coxsackievirus/adenovirus receptor (CAR) required for efficient binding of adenovirus to target cells may influence experimental results.
However, both males and females less than 3 weeks of age or over 40 weeks of age are equally susceptible, indicating that age-related changes in sex-associated hormones may influence experimental myocarditis susceptibility (Grodums and Dempster 1959a; Lyden et al. 1987a).
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Finally, the form of the micronutrient used in supplementation media may also influence experimental results.
Some species of experimental animals retain NCs throughout life, whereas others lose them during development and ageing, which may considerably influence experimental outcomes.
Altered acoustic or visual perception, as well as habituation or sensitization play an important role in fear conditioning and extinction and may substantially influence experimental findings.
The inconsistency between these different 'background matrices' may alternately influence experimental outcomes due to differential target recovery and co-purified inhibition effects.
Willekens et al. [ 204] estimated that the median organ dose in mice from a standard micro-CT scan is about 40 cGy and this may influence the experimental outcome, but adaptation of the scan protocol allows accurate imaging without the risk of interfering with the experimental outcome of the study.
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