Reasons for this discrepancy may include rare variants, structural variants, gene-environment (GxE) interactions, and gene-gene (GxG) interactions.
Such sources may include common variants with limited effect size, rare variants with higher effect sizes, structural variations, or even more complicated mechanisms such as epistatic, gene-environment and epigenetic interactions.
As with other NGS-derived callsets, directly extrapolating sensitivity from this dataset is likely an overestimate (because the dataset may not include variant types/regions that are difficult to sequence).
These nonsense SNPs may include pathological variants that have not yet been reported.
A viral quasispecies emerges within the infected host that is composed of a spectrum of mutants that may include biological variants such as immunologic escape mutants, faster and slower growing viruses, defective viruses, and drug resistant and sensitive viruses [4] [7].
These variations, which cause changes in the length of a polypeptide or which determine whether a protein is translated, may include pathological variants that have yet not been reported.
SNPs identified for an association test of a multiple phenotype may include genetic variants more likely affecting each of single traits which should be excluded.
It may include normal variants of growth, such as familial short stature and constitutional delay of growth and puberty, both characterized by achievement of an adult height within the range of mid-parental height [ 21].
Collectively, our current understanding of the genetic basis for individual variations in opioids responses suggests a strong contribution of other untested variables that may include genetic variants outside of the OPRM1 locus or/and the existence of the other functional SNPs within the OPRM1 gene locus and possibly within other yet undiscovered functional elements of the gene.
Although the fundamental mechanisms of gametocyte immune evasion are still largely unknown, candidate molecules that may be involved include variant antigens encoded by multigene families in the P. falciparum genome, such as the PfEMP1, STEVOR and RIFIN proteins.
