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Although we cannot exclude the possibility that the ZFNs may have cleaved unknown off-target sites, such undesired mutations can subsequently be easily excluded from the genome of the carrier animals by backcrossing to the parental strain or another background strain.
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Rivals have cleaved to the positions of their minority constituencies.
"With this banner you have cleaved us from our country Syria," Abu Moayad said.
Investors have cleaved that value by more than half ahead of the company's initial public offering, likely due to the overall weakness of the company's financials.
Most reactions, to be honest, have cleaved sharply along whether you're Russian or not.
The remainder of the scFv molecule may have been cleaved away by proteolytic activity.
Such weak regions may have been cleaved more frequently during our fragment library preparation, resulting in an absence of high-throughput sequencing data across these regions.
This suggests that transfected dsRNAs may have been cleaved and presumably generated short interfering (si RNAs which were not shown on the gel.
However these results favoring a primer position effect were based upon analysis 24 hrs post treatment, a time point at which abundant mRNA may have been cleaved by the argonaute protein, but not yet degraded by exonucleases.
The N-terminal extended sequences of atp6 in Rhazya may have been cleaved by genomic recombination activities upstream of the protein gene, but remnants of these extended sequences were not detected due to high sequence divergence and frequent recombination activities.
Not all PASRs necessarily overlap the TSSs, suggesting that at least some of these RNAs are not abortive Pol II products, but rather processed RNAs, some of which may have been cleaved and perhaps recapped to serve as yet unknown functions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com