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These results indicated that MSX2 represents an hematopoietic NKL family member which is downregulated during T-cell development and may functionally substituted by oncogenic NKLs.
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Because Gln in Fam20A lacks a negatively charged side chain, it may not functionally substitute the Glu in Fam20C.
A variant of the latter possibility is that stk33 may have become dispensable, because it has been functionally substituted by a non-orthologous, but functionally analogous kinase in neognaths, a process called non-orthologous gene displacement (NOD).
A simpler explanation may be that the R to M mutation was functionally substituted by the K present further down the loop.
Often the promoter boxes are functionally substituted by the transcription activation factor binding sites [ 4].
This defect can be functionally substituted by the N-terminus of Sir3p, which shares sequence similarity with the Orc1p N-terminus.
The authors added four more sequences to their alignment and claim that Giardia is the only organism in which the arginine is functionally substituted by a lysine.
5'NTR sequences may be involved in both RNA replication and translation, and thus the HCV 5'NTR sequences in these chimeric replicons may be required to functionally substitute for GBV-B sequences in providing critical replication signals.
Taken together, and in consideration that Drosophila TDP-43 (TBPH) can functionally substitute for human TDP-43 in functional splicing assays [14], all these reports confirm that Drosophila may represent a highly suitable animal model to investigate TDP-43 functions both in normal and disease conditions.
This may prove to be a useful measure in situations where constructing a GFP fusion that can functionally substitute for the native gene is difficult or impossible.
Furthermore, LUH interacts with SEU in a yeast two hybrid assay suggesting that SEU-LUH complex could functionally substitute for the SEU-LUG complex to mediate repression at target gene which may possibly explain the redundant functions [ 19].
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