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This implies that deep evolutionary history may create constraints on the evolutionary potential of extant species (Ackerly 2003; Kimura 2004; Donoghue 2008; Matzkin et al. 2009).
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These first four rules create constraints that all candidate tracers should obey.
In countries with high levels of external debt, the need to conserve funds for repaying external creditors, perhaps by initiating or increasing user fees for health and education, may create a further constraint.
This is an important constraint that may create difficulties in transplantation experiments such as those where an artificial Mycoplasma genome has been synthesized, using Saccharomyces cerevisiae as an intermediary host (Gibson et al., 2008): it could well be that the resulting folding of the chromosome makes it unreadable by the receiving Mycoplasma machinery.
If both the variables were continuous, we would have ended up having a quadratic problem, which may create issues with convexity if the quadratic terms appear in constraints.
We suggest that domains of open chromatin may create an environment that facilitates transcriptional activation and could provide an evolutionary constraint to maintain clusters of genes together along chromosomes.
Finally, although movement of health personnel may create opportunities for remittances to developing countries, the resultant brain drain is a serious constraint for national health system development.
That may create a long-term problem.
But that may create its own problems.
That in itself may create new realities.
This may create inequality in the country.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com