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We constructed a neighbor-joining tree of all sequences (including artefactual sequences) with the K80 [ 40] model in PAUP 4.0 [ 41] to ensure that each major clade contained at least one non-artefactual sequence and be confident that artefactual sequences represent sequences that may contain amplification, cloning, or sequencing-induced errors.
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CNVs often occur in human cancers and the compositions of CNVs may contain deletion, amplification, deletion plus amplification, multiple alleles and complicated locus.
In addition to higher read depth, reads from PCR amplicons have more uniformly defined ends, more PCR duplication, and may contain more amplification bias.
Nonrespiratory specimens, especially pleural fluid, bone marrow, pus, and tissue biopsy specimens, may contain inhibitors of amplification (22, 23 ).
These observations suggest that centrosomes with structural amplification may contain high levels of γ-tubulin proteins.
This region, like other "hot spots" for gene amplification, may contain several genes that contribute to cell transformation.
Moreover, certain samples of animal origin may contain various inhibitors of PCR amplification [ 22].
According to the Beverley's model for explaining DNA amplification phenomena, the Leishmania genome should contain amplification-prone cassettes [ 30].
Forensic practice sometimes requires the individualization of samples that may contain only highly degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification.
Sequence analysis confirmed the amplification of the intergenic region between ubi and rib genes thus demonstrating that the transcript may contain the sense sequences of the four internal genes and the antisense sequences of the two flanking genes.
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