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This suggests that most RNase IIIs can tolerate a three-way junction and may cleave substrates with less than 16 consecutive base pairs.
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After removal of the propeptide domain, the active site becomes available to cleave substrates.
Proteases may cleave small synthetic substrates whereas their access to physiological proteins in their native form can be somewhat hindered [ 23].
SASPase may cleave other as yet unidentified substrates, in addition to profilaggrin, resulting in decreased SC hydration via abnormalities in the intercellular lamellar lipids barrier, the diffusion path length and/or the composition of non-filaggrin-derived NMFs.
They may cleave the chitin or other substrate molecules randomly or a single residue from the non-reducing end of the chitin molecule.
However, in a physiological situation where multiple Ub chain linkages are often mixed, substrate-targeted DUBs may cleave additional Ub chain types.
PHEX has no known natural substrate, although it has been reported that it may cleave FGF-23, a member of the fibroblast growth factor family that inhibits renal tubular phosphate transport [ 12].
Next, we addressed the question of whether MCMJR1 cleaves substrates within their TMD.
They are cysteine proteases that cleave substrates after specific aspartate residues.
Rhomboid proteins are a family of serine proteases that cleave substrates within transmembrane domains[ 69, 70].
Typically, however, MMPs have a poor substrate specificity and will cleave many substrates in in vitro assays.
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