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These two examples demonstrate how important is to consider that ExMVs released during cell purification may change cell surface phenotype.
If the cells have phenotypic plasticity, they may change cell division strategies in response to the environment in a short duration.
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For instance DNA modification in a cell may change the cell in a tumor cell which then duplicates much faster than a normal one changing the overall scenario both at cellular and at tissue level.
The copy numbers of mitochondrial DNA (mtDNA) in cells may change during cell growth and differentiation [ 13]. mtDNA is highly susceptible to oxidative stress that leads to mitochondrial dysfunction [ 3, 14].
We hypothesize that the immobile epitope fraction may change with cell adhesion, and we find that such a model is more consistent with the data, although discrepancies remain.
These different mechanisms of isoform diversification may co-exist for the same microRNA and the isoform profile of each microRNA may change between cell types.
One possible mode of action for ZTDO may be its ability to change cell membrane permeability, thereby indirectly affecting the functions of the glia-associated neurons.
The surface-accessibility of wall-associated proteins, which serve as receptors for binding of plasminogen in C. neoformans, may change as cells age during culture, in vitro.
In the nucleus of eukaryotic cells, chromosomes occupy distinct territories whose position may change during the cell cycle or cell differentiation [ 1– 4].
For example, a precursor cell may change to a differentiated cell type after receiving a transient signal in which the underlying genetic network moves from one stable steady state representing the precursor cell type to another stable steady state representing the differentiated cell type.
Also, activity or availability of host cell factors required for virus replication may change with the cell cycle [ 31] or with metabolic shifts [ 28, 31] at higher cell densities.
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