Of primary interest to the present study is the differential flow instability, which may cause amplification of small amplitude inlet perturbations of concentration, temperature, and flow rate into large temperature excursions.
Although we know that the geological and topographic conditions and the distance to the source fault of the earthquake may cause amplification and attenuation of the seismic shaking in different site, the coefficient of the amplification and attenuation is very difficult to be determined.
None of this may be important for procedures such as PCR as long as some of the DNA of the animal is intact; however it may cause amplification-variations due to the highly variable amounts of DNA present.
In addition, stochastic processes in primer binding and amplification during the initial stages of MDA may cause uneven amplification across the genome, leaving some regions extremely abundant in sequencing libraries while others have little or no representation [2], [8], [9], [11].
Such as miR-182 is one member of a miRNA cluster in a chromosomal locus (7q31-34) frequently amplified in HCC [ 13], the amplification may cause the up-regulation of miR- 182.
Also, AP amplification loop may cause detrimental activation no matter the initial trigger [54].
The Orders to which the Japanese eel, broadbanded thornyhead, and the Japanese pilchard belonged were not involved in primer design, which may cause the slight difference in amplification success between the former and later fish groups.
The rare alternative mechanisms that may cause imatinib resistance include KIT amplification and loss of tumor KIT expression [ 27].
Our simple experimental procedure is also the basis of the high degree of sensitivity since it avoids complicated mRNA extraction and processing procedures before and during amplification, which may cause mRNA degradation or loss.
33, 34 In the current and our previous studies, 12 the strict selection of cancer cells with a sufficient concentration of EGFR-TKIs may prevent the acquisition of conventional resistance mechanisms, such as T790M or MET amplification, and may cause cancer cells to acquire stem cell-like properties.
Since PCR amplification of adapter-tag fragments may cause a distortion in transcript profiles due to preferential amplification of a subset of tags, we evaluated how the number of PCR cycles affects tag profiles.
