The reduction of the spreading of cells that overexpress BCL2 suggests that there may be abnormal actin polymerization close to the the plasma membrane.
This result suggests that parasites treated with Aspirin are less efficient in capping formation because Aspirin may be affecting actin polymerization and therefore those functions that depend on the rearrangement of the cytoskeleton.
The difficulties in visualization of β- and γ-actins in the cell nucleus using microscopic techniques may be due to actin modifications, binding to other proteins or different conformation (Steinmetz et al. 1997; Pederson and Aebi 2002; Bettinger et al. 2004; Zhong et al. 2010).
They may be precursors to actin stress fibers that regulate contractility in cells.
Additionally, several studies indicated that MTSS1 may be due to actin binding, reorganisation or changes in cell adhesion or tyrosine phosphorylation upon loss of MTSS1 [ 27].
Monomeric actin is a core component of the INO80 and SWR chromatin remodeling complexes and the NuA4 histone modification complex, and our results suggest these complexes may be sensitive to actin stoichiometry.
Significantly, Lifeact-mEGFP labels both G- and F-actin, so some of the signal in the center of the tube may be G-actin [31].
Hence, we suggest that the decrease in synaptic vesicle density following synapsin I + II perturbations (Rosahl et al. 1995; Gitler et al. 2004; Bogen et al. 2006) may be F-actin dependent.
As for CRP proteins, the tobacco NtWLIM1 binds F-actin and may be involved in actin cytoskeleton stability.
