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Since the short read sequences are transcript derived, differential expression between homeologs within varieties must also be considered because this may affect sampling of different alleles in the sequence space.
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However, prolonged exposure of the extracts to high pH may in turn lead to degradation of the matrix and this may affect sample cleanup.
Such long-term storage may theoretically affect sample concentrations.
Also, it is shown that uncertainties in structural strength may affect directional sampling in load space.
CCA plots of sampling sites and environmental factors suggested that TS may control the taxonomic composition of G4 and G5 samples, while saline water saturation may affect L1 samples.
First, a convenience sample lacks randomness; thus, selection bias may affect the sample, reducing the generalizability of the results.
Fluid loss due to evaporation may affect the sample since MA works in an open environment also need for periodic recalibration to eliminate the effect of drifting baseline.
Other predicted variables, including the yearly event rate (which may differ based on the patient population) and patient dropout rate from the study, may affect the sample size and duration of the trial.
This finding requires further study, nevertheless it may be reflective of the unavoidable systematic biases that may affect small sample size case-control candidate gene studies; in this respect, the 'agnostic' genome-wide approach that we employed using two population-based samples constitutes an improvement.
As both genetic (effect of population size) and statistical (sample size) sampling may affect the results obtained (see Weir 1996), sample size effects need to be taken into account in the analyses.
Another possible limitation is nasal self-swabbing; since subjects have to swab their own nostrils, this may affect the quality of sampling.
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