The SNP substitution may affect protein function compensated by redundant functionality outside the affected protein in the larger physiological system.
Amino acid change upon Thr21Met polymorphism may affect protein folding efficiency or structure.
"We can't look at them all individually, [so] we use special tools to identify mutations that may affect protein function".
It is possible that the addition of the affinity tag may affect protein function and pH optimum, as it has previously been observed for other proteins (Thielges et al. 2011).
Similarly, principal component analysis indicated that two amino acid substitutions (E264K and V231G) occupied less conformational clusters of folded states as compared to the WT and were found to be destabilizing (may affect protein function).
Thus, it is essential to develop a detailed understanding of how nanostructured surface characteristics, such as atomic-scale topography, surface energy, and chemical structure may affect protein adsorption, structure, function, and stability.
The present study reveals changes in the folding landscape of hPGK1 upon mutation that may affect protein folding efficiency and stability in vivo, also suggesting that native state stabilizers and protein homeostasis modulators may help to correct folding defects in hPGK1 deficiency.
We analyze the properties of the clusters we find and discuss how some of these clusters may affect protein function.
Beyond these differences in amino acid sequences, which may affect protein function, the regulation of NHEJ genes appears to be different in B. pumilus.
The gene CCDC91 in this locus may affect protein sorting and membrane trafficking through interacting with GGAs (Golgi-localized, Gamma ear-containing, ARF-binding proteins) [22].
Though there are mechanisms by which synonymous mutation may affect protein function, they are more likely to be effectively neutral than non-synonymous mutations [14].
