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We showed that sFlt-1 does not interfere with the current R&D pan-VEGF ELISA, ruling this out as an explanation for the discrepancy, but other proteins, such as soluble VEGFR2, may affect binding.
Given the findings of Saven et al., [23] it was essential to consider possible structural deviations or fluctuations in the α1β1 loop region that may affect binding of such inhibitors.
Panning with intact tissue presents additional relevant cell targets while accounting for subtle features of the tissue microenvironment that may affect binding [15], [22] [24].
High sequence divergence may affect binding efficiencies and thus lead to erroneous results regarding transcript abundance.
Given this conserved sequence it seems likely that this change may affect binding of Engrailed to the transcriptional repressor Groucho [ 52, 53].
The observation that the GE target is adjacent to the Rif target raises the possibility that binding of GE to RNAP may affect binding of Rif to RNAP.
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Some of the mutations discovered are in or near the FAD binding site and hence may affect FAD binding.
Indeed it's quite possible that CgrA binding to these "silent sites" may affect the binding of other transcription factors that have roles at later stages of development, which are not analyzed in our study.
The non-binding domain of the aptamer may affect the binding affinity of the aptamer-target complex.
Methylation of the cytosine base changes the topology of the major DNA groove, which may affect the binding of many transcription factors and DNA-binding proteins.
The conservative substitution of glutamate residue (Glu 95), involved in the binding site, with an aspartate residue in the DUF family, may affect the binding affinity or rate of the reaction.
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