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In addition, antibacterial activity against Staphylococcus sp. (NCBI-Accession: KC688883.1) and Klebsiella sp. (NCBI-Accession: KF649832.1), showed maximum zone of inhibition of 13 mm and 12.3 mm, respectively, at 10 μg/mL of AgNPs.
The maximum zone of inhibition was observed in K. planticola followed by B. subtilis (Figure 8).
The maximum zone of inhibition showed against S. aureus with zone diameter of 9 mm.
The maximum zone of inhibition occurred at 50 μl concentration of silver nanoparticles.
The maximum zone of inhibition was found in the A. flavus followed by A. niger.
Then, the maximum zone of inhibition were observed and measured for analysis against each type of test microorganism.
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P. aeruginosa 3 was the most sensitive strain as indicated by the formation of maximum zones of inhibition (mm) in the presence of all four types of lyso-Ag NPs applied.
The antibacterial activity of AgNPs synthesized with Rosmarinus officinalis extracts was tested against gram-positive bacteria, and the maximum zones of inhibition at dosages of 20, 40, and 80 mg/disk were 21.52, 30, and 31.2 mm, respectively, against S. aureus and 13.4, 15.63, and 16.21 mm, respectively, against Bacillus subtilis [85].
The sensitivity of aromatic carbon signals to the stereosequences was found to depend mainly upon the carbon ability to extend into the maximum shielding zone of the neighbouring phenyl rings and carboxyl oxygens.
The sensitivity of both aromatic and methyl (those attached to the aromatic ring) carbon signals to the stereosequences depends mainly on the proximity of these carbons to the maximum shielding zone of the neighbouring conjugated blocks formed by the aromatic ring and the pyran cycle.
This is because of the position of opening under the applied load and in the maximum shear zone of the beam.
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