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Samples above the maximum were diluted 1 : 2 in the reagent diluent provided with the kit and retested.
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Of the 1344 samples of the 1995 2005 time series, 724 were diluted to a maximum dilution of 1 204800, 17 to a maximum dilution of 1 3200, and 553 to a maximum of 1 800.
The compounds which were found satisfactory by the above two methods at a maximum concentration of 50 µg/ml were diluted further to concentrations viz., 25, 12.5, 6.25, 3.125 and 1.56 µg/ml respectively.
Where required, samples were diluted so that maximum absorbance of the dye solution remained under 1.0 so that measurements would behave according to Lambert Beer Law.
Homogenate samples close to, or exceeding, the maximum point of the standard curve were diluted and assayed again.
When diets were diluted from the maximum concentration of 15% sugar and yeast, chico-null and wild-type genotypes robustly increased lifespan, but chico mutants were longest lived upon diet with 6.5% sugar and yeast, while wild-type were longest lived upon diets with 8% of these nutrients.
If the maximum concentration in the calibration standards was exceeded, then samples were diluted gravimetrically to within the targeted analytical range.
Compounds were diluted in the running buffer at a maximum concentration of 1 mM and then threefold diluted to a concentration series ranging from 1 mM to 457 nM.
Sample were diluted at least 5 fold into loading buffer and a maximum of 2 µl of a translation reaction was used per well to avoid overloading.
Thawed serum samples were diluted (1 1) with deionized sterile water and vortexed at a maximum of 2700 rpm for 10 s to homogenize the samples.
Bold proposals were diluted.
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