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The use of appropriate cell substrates and methods for culturing these cells for production of vaccines represents a critical factor affecting the maximum viral yield, genetic stability, immunogenicity, and ultimately vaccine safety and economic feasibility.
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Conclusion: when aiming for high viral yield, BLV should be harvested between days 6 and 8 after passage, when viral shedding is at its maximum.
The multiple-cycle viral yield assay provides two quantities: the viral titer growth rate, and the viral titer decay rate.
Analysis of viral yields on plaque titration revealed that the viral yield reached a value of 3 to 6 106 FFU/mL at 24 h.
One parameter can be determined immediately from the multiple-cycle viral yield results.
Analysis of total viral yield in bronchi tissue homogenate was performed as described previously [49].
Single-cycle viral yield experiments have provided qualitative confirmation of these findings.
Viral load was included in the analysis as the maximum viral load attained during an incident infection for each defined viral group.
Viral yield did not differ between TCC-SUP and 293 cells infected with Ad5WS1.
For total viral yield in mouse muscles, a Mann–Whitney t test was used.
Studies indicate that up to 95% adherence is required to achieve maximum viral load suppression [ 3].
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