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Cell culture carried out in the bioreactor exhibited a maximum viable cell concentration (Xmax) of 1.0 × 106 cells/mL with μmax = 2 × 10−2 h−10−2
By applying the optimized feeding regime, a maximum viable cell density (VCD) of 6.45 × 106 cells/mL and volumetric antibody production of 632 mg/L were achieved in a 2 L-B.Braun bioreactor.
These engineered cells reached higher maximum viable cell density and extended viability compared with negative control and parental CHO cells in batch cell cultures which resulted in the 53.8% and 41.6% increase of integral viable cells.
Maximum viable cell concentration obtained was significantly different (P < 0.05) at all of the three feeding rate.
Maximum viable cell concentrations, viable cell yield and productivity obtained were significantly different (P < 0.05) at all of the three agitation speeds studied.
There were significant differences (P < 0.05) in maximum viable cell concentration, yield and productivity for both fed-batch fermentations with and without resin addition.
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Although glucose concentration in the culture at feeding rate of 0.008 L/h was similar to the culture at 0.015 L/h, however, maximum viable cells concentration obtained at constant feeding rate of 0.008 L/h was significantly (P < 0.05) lower (Fig. 5a).
For both the days, the maximum number of viable cells were seen on 100-nm nanoporous surfaces.
As shown in Fig. 5a d, the buffer treated cells (0 μM) had the maximum number of viable cells showing green color.
After a short Lag phase of about 5 h, we witness an exponential phase of bacterial growth and maximum number of viable cells was attained after a period of 45 h (Figure 3).
Viable cell counts reached a maximum of 105 colony forming units/ml at lower temperatures of −10 and −15 °C, while 108 colony forming units/ml were obtained at optimal growth temperatures (Figure 1; Supplementary Figure S1).
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