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Time to peak (TTP) was calculated by measuring the time taken to reach maximum twitch tension.
The length of the muscles was adjusted for maximum twitch tension.
The length of the muscles was adjusted for maximum twitch tension and the muscle and nerves were kept moist with saline throughout the recordings.
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Optimal length, i.e the length producing maximal twitch tension, was determined.
Maximum twitch and tetanic tension, time to peak, and half-relaxation time values were measured using a computer and appropriate software (Scope).
The maximum twitch and tetanic tension was measured using a computer and Picoscope software (Pico Technology, Cambridgeshire, UK).
Muscle length was appropriately adjusted in order to produce maximal single twitch tension (optimal length), through a micromanipulator allowing motion on the 3 axes (Prior, England).
Muscle length was adjusted so as to produce maximal single twitch tension (optimal length), through a micromanipulator allowing motion on the 3 axes (Prior, England).
Initial length, weight, maximum twitch height, predrug resting tension and predrug twitch height of muscle bundles did not differ between groups.
Muscle bundles used for the IVCT and CPA-IVCT did not differ regarding to length, weight, maximum twitch height, predrug resting tension and predrug twitch height (Table 1).
When quercetin (10 μg/mL) was incubated alone, there was a maximum decrease of muscle twitch tension of 20 ± 0.5% in 120 min recordings, although not significative, P > 0.05 compared to the control Tyrode).
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