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Muscle bundles used for the IVCT and CPA-IVCT did not differ regarding to length, weight, maximum twitch height, predrug resting tension and predrug twitch height (Table 1).
Initial length, weight, maximum twitch height, predrug resting tension and predrug twitch height of muscle bundles did not differ between groups.
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There is a transient decrease in twitch height, followed by a facilitatory phase and finally a progressive blockade.
The EHTs showed a steep increase in twitch tension from 0.47 ± 0.02 to 0.91 ± 0.02 mN/mm2 as they were stretched at a constant rate (2.67% per min) from 86% to 100% of the length at which maximum twitch force was exerted.
Maximum twitch and tetanic tensions were similar in WT and TG but force at subtetanic frequencies (30 and 50 Hz) was reduced in TG SOL.
The ED95 of cisatracurium is the dose required to produce a 95% twitch height depression for the first twitch response of the adductor pollicis muscle after stimulation of the ulnar nerve, and is approximately 0.05 mg.kg-1 of body weight during anesthesia with hypnotics and opioids (thiopental/midazolam/fentanyl) [12, 13].
The muscle was adjusted to an optimum length (Lo) that produced the maximum twitch force (Pt).
A series of isometric twitches were used to set the length of the muscle to that at which maximum twitch force was produced (L0).
At the start of the experiment, the muscle was set to the optimum length L0 that produced maximum twitch force.
Single twitch contractions were recorded, and maximum twitch force (Ft) was calculated.
Time-to-peak-twitch force, half-relaxation time, maximum twitch force and maximum tetanic force were determined.
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