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For this reason we have used the terms maximum turnover rate and S0.5, corresponding to the substrate concentration leading to half of the maximum turnover rate, to describe the kinetic behavior of the different HSL enzymes.
The S0.5 value was 0.73±0.13 mM and the maximum turnover rate was 2.3±0.11 U/mg (Figure 8F and Table 2).
The maximum turnover rate for TO was 1.3±0.028 U/mg for wild type and 0.42±0.015 U/mg for AVA-AA.
Analysis of the kinetic behavior of the wild type enzyme against TO revealed that not only did phosphorylation increase maximum turnover rate, but it also increased S0.5 compared to the non-phosphorylated enzyme.
In conclusion, we have demonstrated that activation of human HSL against lipid substrates in response to phosphorylation by PKA in vitro is governed by Ser649 and Ser650 and that this activation involves alterations in the kinetic behavior of the enzyme with increases in both maximum turnover rate and S0.5.
Glucokinase, one of the hexokinase isozymes, is expressed in the liver and the pancreas, and requires a high concentration of glucose to reach the maximum turnover rate.
Similar(51)
and maximal turnover rate.
Thus, U reductive removal rates measured in ligand-amended media – which were slower than rates in control media – were not constrained relative to each other by the ceiling imposed by the maximum enzymatic turnover rate.
Compared with control siRNA-treated cells, p32 siRNA-treated cells showed a significant reduction in the ATP turnover rate and maximum respiration in addition to decreased basal respiration.
Minimum, maximum, and average turnover rates for each cadre and facility-type were examined.
Litterfall in coniferous forests is assumed to be constant for each day of the year (Pedersen and Bille-Hansen, 1999), with the daily rate reset each year depending the previous year's maximum LAI and a species-specific turnover rate.
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