Thus, even if we assume that these two mRNAs require eIF4G only at the step of 43S attachment to achieve their maximum translation rates, it would have been very difficult to detect a 10% decrease in the levels of their free 48S complexes with the techniques employed in the previous study [ 8].
Furthermore, the two factors are connected so that a protein's robustness to misfolding dependent on structural functional constraints, to a large extent, determines the maximum allowable translation rate of the given protein.
Using mRMR method, we ranked and analyzed the top 500 relevant features to translation rate with Maximum Relevance Minimum Redundancy method.
In the absence of sex chromosome dosage compensation, highly expressed genes at or near the point of maximum translation will exhibit a strong dosage effect; the single female gene copy cannot reach the transcription rate of two copies in male.
More specifically, to identify the most important features regulating translation rates under different conditions, we applied maximum relevance & minimum redundancy and incremental feature selection to select the important features for predicting the translation rate in rich and starvation conditions, respectively.
Liu, Kaplan et al. found that the maximum force generated by a ribosome is only just enough to unwind these mRNA structures, making the translation rate highly sensitive to the existence of such structures, and the structures themselves of high importance for regulating transcription.
The parameters nominated by this analysis are: mRNA transcription rate; mRNA degradation rate; protein translation rate.
The translation rate prediction model could become a useful tool for annotating the translation rate of mRNAs in large-scale.
We hence use three assays to approximate translation rate.
Gene expression was described by transcription and translation rate equations.
